Render Target: STATIC
Render Timestamp: 2025-03-20T11:22:30.794Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:08:51.675
Product last modified at: 2025-01-01T09:04:34.526Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

LPP (8B3A11) Mouse mAb #3389

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M Hm Mk
    SENSITIVITY Endogenous
    MW (kDa) 75
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Hm-Hamster 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:100
    Immunofluorescence (Immunocytochemistry) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LPP (8B3A11) Mouse mAb detects endogenous levels of total LPP protein.

    Species Reactivity:

    Human, Mouse, Hamster, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a HIS-tagged recombinant fragment of human LPP.

    Background

    LIM-containing lipoma-preferred partner (LPP) belongs to the zyxin family, members of which include LIMD1, ajuba, trip6 and zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex (1). They shuttle in and out of the nucleus where they may function in transcriptional activation (1).
    LPP binding partners at cell-cell contacts include the actin regulator α-actinin (2) and the human tumor suppressor scrib which regulates cell migration and polarity (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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