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REACTIVITY SENSITIVITY MW (kDa) SOURCE
75 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

LRF/Pokemon Antibody recognizes endogenous levels of total LRF (Pokemon) protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro241 of human LRF (pokemon) protein. Antibodies are purified by protein A and peptide affinity chromatography.

Zinc finger and BTB domain-containing protein 7A (LRF, Pokemon, FBI1) is a transcriptional repressor encoded by the ZBTB7A gene that belongs to the POK (POZ and Kruppel)/ZBTB (zinc finger and BTB) family (1). LRF is broadly expressed with elevated expression in a variety of cancers relative to normal tissues, including non-small cell lung cancer, breast cancer, ovarian cancer, prostate cancer, and hepatocellular carcinoma (1-8). Research studies suggest that LRF acts as an oncogene through various mechanisms including repression of the tumor suppressors ARF and Rb, and repression of the cell cycle arrest factor p21Cip1 (9-11). The LRF transcription factor plays key roles during several stages of hematopoiesis including promoting lymphoid progenitor cells to commit to B cell differentiation by repressing T cell-promoting Notch signals, and promoting cell survival during terminal erythroid differentiation through suppression of the proapoptotic factor Bim (12,13).


1.  Lee, S.U. and Maeda, T. (2012) Immunol Rev 247, 107-19.

2.  Apostolopoulou, K. et al. (2007) J Pathol 213, 294-302.

3.  Zhao, Z.H. et al. (2008) Lung Cancer 62, 113-9.

4.  Qu, H. et al. (2010) Cancer Invest 28, 672-8.

5.  Aggarwal, A. et al. (2010) Exp Mol Pathol 89, 140-8.

6.  Jiang, L. et al. (2010) Mol Cancer 9, 318.

7.  Aggarwal, H. et al. (2011) Exp Mol Pathol 90, 226-30.

8.  Fang, F. et al. (2012) Cancer 118, 134-46.

9.  Maeda, T. et al. (2005) Nature 433, 278-85.

10.  Jeon, B.N. et al. (2008) J Biol Chem 283, 33199-210.

11.  Choi, W.I. et al. (2009) J Biol Chem 284, 12633-44.

12.  Maeda, T. et al. (2007) Science 316, 860-6.

13.  Maeda, T. et al. (2009) Dev Cell 17, 527-40.


Entrez-Gene Id 51341
Swiss-Prot Acc. O95365


For Research Use Only. Not For Use In Diagnostic Procedures.
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SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
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LRF/Pokemon Antibody