LRF/Pokemon (D7U2O) Rabbit mAb (BSA and Azide Free) #27432
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
LRF/Pokemon (D7U2O) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total LRF/Pokemon protein. This antibody cross-reacts with a 44 kDa protein of unknown origin.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant LRF/Pokemon protein.
Background
Zinc finger and BTB domain-containing protein 7A (LRF, Pokemon, FBI1) is a transcriptional repressor encoded by the ZBTB7A gene that belongs to the POK (POZ and Kruppel)/ZBTB (zinc finger and BTB) family (1). LRF is broadly expressed with elevated expression in a variety of cancers relative to normal tissues, including non-small cell lung cancer, breast cancer, ovarian cancer, prostate cancer, and hepatocellular carcinoma (1-8). Research studies suggest that LRF acts as an oncogene through various mechanisms including repression of the tumor suppressors ARF and Rb, and repression of the cell cycle arrest factor p21Cip1 (9-11). The LRF transcription factor plays key roles during several stages of hematopoiesis including promoting lymphoid progenitor cells to commit to B cell differentiation by repressing T cell-promoting Notch signals, and promoting cell survival during terminal erythroid differentiation through suppression of the proapoptotic factor Bim (12,13).
- Lee, S.U. and Maeda, T. (2012) Immunol Rev 247, 107-19.
- Apostolopoulou, K. et al. (2007) J Pathol 213, 294-302.
- Zhao, Z.H. et al. (2008) Lung Cancer 62, 113-9.
- Qu, H. et al. (2010) Cancer Invest 28, 672-8.
- Aggarwal, A. et al. (2010) Exp Mol Pathol 89, 140-8.
- Jiang, L. et al. (2010) Mol Cancer 9, 318.
- Aggarwal, H. et al. (2011) Exp Mol Pathol 90, 226-30.
- Fang, F. et al. (2012) Cancer 118, 134-46.
- Maeda, T. et al. (2005) Nature 433, 278-85.
- Jeon, B.N. et al. (2008) J Biol Chem 283, 33199-210.
- Choi, W.I. et al. (2009) J Biol Chem 284, 12633-44.
- Maeda, T. et al. (2007) Science 316, 860-6.
- Maeda, T. et al. (2009) Dev Cell 17, 527-40.
Limited Uses
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