LRP1 (E2Q7S) Rabbit mAb #26387
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 85 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
LRP1 (E2Q7S) Rabbit mAb recognizes endogenous levels of total LRP1 β subunit.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu4488 of human LRP1 protein.
Background
Low density lipoprotein receptor related protein 1 (LRP1) is a type I transmembrane receptor that mediates the endocytosis of various ligands (1). LRP1 plays important roles in lipid homeostasis, signaling transduction, embryonic development, and glucose metabolism (2-6). In addition, LRP1 regulates APP processing and facilitates the clearance of beta-amyloid (7-9). This finding makes LRP1 a potential therapeutic target for Alzheimer’s disease. LRP1 preprotein is proteolytically processed by furin to generate a 515 kDa extracellular α subunit and a membrane-anchored 85 kDa β subunit, which together form the mature receptor (10).
- Lillis, A.P. et al. (2008) Physiol Rev 88, 887-918.
- Herz, J. et al. (1988) EMBO J 7, 4119-27.
- van der Geer, P. (2002) Trends Cardiovasc Med 12, 160-5.
- May, P. and Herz, J. (2003) Traffic 4, 291-301.
- Zilberberg, A. et al. (2004) J Biol Chem 279, 17535-42.
- Hofmann, S.M. et al. (2007) J Clin Invest 117, 3271-82.
- Cam, J.A. et al. (2005) J Biol Chem 280, 15464-70.
- Bu, G. et al. (2006) Ann N Y Acad Sci 1086, 35-53.
- Bell, R.D. et al. (2009) Nat Cell Biol 11, 143-53.
- Willnow, T.E. et al. (1996) Biochem J 313 (Pt 1), 71-6.
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