Lung Cancer RTK Antibody Sampler Kit #79884
Product Information
Kit Usage Information
Protocols
- 2085: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 3197: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 3287: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 3633: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Flow
- 4267: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 4290: Western Blotting, Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin)
- 7074: Western Blotting
- 8198: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 9740: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 14556: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow
Product Description
Specificity / Sensitivity
Each antibody in the Lung Cancer RTK Antibody Sampler Kit detects endogenous levels of its target protein. FGF Receptor 1 (D8E4) XP® Rabbit mAb may slightly cross-react with overexpressed FGF receptor family members. IHC staining for ROS1 (D4D6®) Rabbit mAb may be observed in ROS1 rearranged lung carcinomas, macrophages/giant cells, reactive type II pneumocyte hyperplasia, and the epithelium in areas of bronchiolar metaplasia. Staining of unknown specificity has been observed in cholangiocarcinoma, hepatocellular carcinoma, and kidney tissues. HER2/ErbB2 (D8F12) XP® Rabbit mAb may cross-react slightly with other overexpressed RTKs. EGF Receptor (D38B1) XP® Rabbit mAb does not cross-react with other proteins of the ErbB family. Species cross-reactivity for IHC-P, IHC-BOND, and IF-IC is human only. EGFR (L858R Mutant Specific) (43B2) Rabbit mAb may cross-react with wild-type EGFR and other HER family members when highly overexpressed. Careful titration of this antibody may be required to obtain optimal specificity.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding E746-A750del and L865 mutant sequences of human EGFR, Pro320 of human Ret protein, and residues near the carboxy terminus of human Met protein, or recombinant proteins specific to the carboxy terminus of human FGF receptor 1, human ALK, human ROS1, the amino terminus of human HER2/ErbB2, and the cytoplasmic domain of human EGF receptor.
Background
Lung cancer is the leading cause of cancer-related mortality worldwide (1). It is generally divided into two broad histological classifications: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). NSCLC comprises about 80-85% of all lung cancers. Receptor tyrosine kinases (RTKs) are essential components to cellular signaling pathways and are often overexpressed or otherwise dysregulated by genetic mutations, fusion, or gene amplification (2,3). RTKs are generally activated by receptor specific ligands, leading to autophosphorylation and the subsequent recruitment of downstream signaling proteins. The most common RTK amplification in NSCLC is that for epidermal growth factor receptor (EGFR). Also, two of the most common mutations in EGFR include an exon 19 deletion, E746-A750, and a point mutation L858R (4,5). In addition to EGFR, several other RTKs may become aberrantly activated in NSCLC, including ALK, ROS1, HER2/ErbB2, Met, Ret, FGF Receptor 1, and NTRK (6). Specific tyrosine kinase inhibitors (TKIs) have been part of the arsenal of treating the disease and so analyzing the expression and mutational status of these receptors plays an important role in personalized treatment.
- Sung, H. et al. (2021) CA Cancer J Clin 71, 209-249.
- Reinmuth, N. et al. (2006) Int J Cancer 119, 727-34.
- Du, Z. and Lovly, C.M. (2018) Mol Cancer 17, 58.
- Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
- Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.
- Rebuzzi, S.E. et al. (2021) Int J Mol Sci 22, 2625.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.