Render Target: STATIC
Render Timestamp: 2024-11-05T10:16:44.758Z
Commit: 57f6e368eba1a427377652f2ad915d45d7f340a4
XML generation date: 2024-08-01 15:31:11.917
Product last modified at: 2024-11-01T14:30:11.986Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Ly-6G (E6Z1T) Rabbit mAb #87048

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 20-40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    IHC Leica Bond 1:300 - 1:1200
    Immunohistochemistry (Paraffin) 1:75 - 1:300

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #75028.

    Protocol

    Specificity / Sensitivity

    Ly-6G (E6Z1T) Rabbit mAb recognizes endogenous levels of total Ly-6G protein. This antibody does not cross-react with Ly-6C1, Ly-6C2, Ly-6F, or Ly-6I. Non-specific staining was observed in mouse gastrointestinal epithelium by immunohistochemistry.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with mouse Ly-6G recombinant protein.

    Background

    The Ly-6 complex is a series of genes found on chromosome 15. These genes code for a number of different proteins that can be used as surface markers. The family members vary in their biologic expression and have been shown to be involved in cell signaling and cell adhesion (1). The structure of these proteins includes a motif known as the LU domain that has three loops comprised of disulfide bonds. These bonds are formed by 8 to 10 cysteines that can cause differences in the length of the loops as well as the sequences at each tip (2,4). There are 11 known Ly-6 genes on murine chromosome 15 that code for different proteins. These family members, excluding secreted Ly6/Plaur domain containing 1 coded by the Slurp1 gene, are attached to the cell surface by a GPI anchor near the C terminus. The structure of these proteins may play a role in transmembrane interactions, and downstream signaling cascades (1,2).

    Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5). 

    Ly-6G is used as a marker for mouse neutrophils (2,3). It is also expressed by mouse polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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