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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LYAR (E6H1A) Rabbit mAb #31468

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  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 48
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LYAR (E6H1A) Rabbit mAb recognizes endogenous levels of total LYAR protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LYAR protein.

    Background

    Ly-1 antibody reactive clone (LYAR) is a zinc finger motif-containing nucleolar protein that is expressed at high levels in male germ cells (1). Research studies have shown LYAR plays a role in the control of embryonic stem cell (ESC) self-renewal and differentiation (2,3). Given its nucleolar localization and oncogenic potential, LYAR has been shown to participate in the regulation of ribosomal gene transcription, rRNA processing, and ribosome biogenesis; processes that many cancer cells and ESCs ramp up in order to support high rates of cell proliferation (4,5). In addition to its role in controlling cell growth and proliferation, LYAR has also been shown to play a role in negatively regulating the host antiviral innate immune response by suppressing the expression of type-I interferons and proinflammatory cytokines (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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