MAGE-C2/CT10 (E7B8D) XP® Rabbit mAb #20058
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:4000 - 1:16000 |
Storage
For a carrier free (BSA and azide free) version of this product see product #16675.
Protocol
Specificity / Sensitivity
MAGE-C2/CT10 (E7B8D) XP® Rabbit mAb recognizes endogenous levels of total MAGE-C2/CT10 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val75 of human MAGE-C2/CT10 protein.
Background
Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3).
Melanoma-associated antigen-C2/Cancer testis antigen 10 (MAGE-C2/CT10) belongs to the type I MAGE family of proteins. In normal human tissues, MAGE-C2/CT10 expression is nuclear and largely restricted to germline cells of the testis (4). At the molecular level, research studies have demonstrated that MAGE-C2/CT10 physically interacts with and enhances activity of the TRIM28 E3 RING ubiquitin ligase (5,6). MAGE-C2/CT10 has also been shown to interact with and regulate the activity of SCF CRL E3 ubiquitin ligase complexes toward substrates that control cell cycle progression, suggesting a role for MAGE-C2/CT10 in malignant transformation (7). Indeed, MAGE-C2/CT10 is aberrantly expressed in testicular germ cell tumors, prostate carcinoma, hepatocellular carcinoma, and melanoma (8-11). Furthermore, MAGE-C2/CT10 is immunogenic in some tumor types and as a result, there is interest in targeting antigenic epitopes presented in the context of class I MHC alleles using engineered TCRs (12,13).
- Caballero, O.L. and Chen, Y.T. (2009) Cancer Sci 100, 2014-21.
- De Smet, C. et al. (1999) Mol Cell Biol 19, 7327-35.
- Gjerstorff, M.F. et al. (2015) Oncotarget 6, 15772-87.
- Zhuang, R. et al. (2006) Cancer Immun 6, 7.
- Doyle, J.M. et al. (2010) Mol Cell 39, 963-74.
- Yang, B. et al. (2007) Cancer Res 67, 9954-62.
- Hao, J. et al. (2015) Oncotarget 6, 42028-39.
- Bode, P.K. et al. (2011) Mod Pathol 24, 829-35.
- von Boehmer, L. et al. (2011) PLoS One 6, e21366.
- Riener, M.O. et al. (2009) Int J Cancer 124, 352-7.
- Curioni-Fontecedro, A. et al. (2011) PLoS One 6, e21418.
- Ma, W. et al. (2011) Int J Cancer 129, 2427-34.
- Straetemans, T. et al. (2012) Clin Dev Immunol 2012, 586314.
Limited Uses
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