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MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb #48086

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Inquiry Info. # 48086

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 135
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MAGI3-Akt3 Fusion Protein (D4D3P) Rabbit mAb recognizes transfected MAGI3-Akt3 fusion protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the fusion site of MAGI3-Akt3 fusion protein.

    Background

    MAGI3-Akt3 fusion was first detected in triple-negative breast cancer tissues in a sequence analysis research study (1). Rearrangement occurs between the genes MAGI3 (membrane-associated guanylate kinase, WW and PDZ domain containing 3) on chromosome 1p and Akt3 on chromosome 1q, leading to a translocation from intron 9 in MAGI3 to intron1 of Akt3. This rearrangement yields an in-frame fusion gene that translates into a MAGI3-Akt3 fusion protein. Overexpression studies show that MAGI3-Akt3 fusion protein leads to the constitutive activation of Akt kinase (1). Further research studies demonstrate that this fusion is not a recurrent event in triple-negative breast cancer (2,3).

    Alternate Names

    AKT serine/threonine kinase 3; AKT3; dJ730K3.2; DKFZp434N0250; KIAA1634; MAGI-3; MAGI3; membrane associated guanylate kinase, WW and PDZ domain containing 3; Membrane-associated guanylate kinase inverted 3; membrane-associated guanylate kinase-related 3; Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3; MGC163281; MPPH; MPPH2; PKB gamma; PKB-GAMMA; PKBG; PRKBG; Protein kinase Akt-3; Protein kinase B gamma; RAC-gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; RP4-730K3.1; serine threonine protein kinase, Akt-3; STK-2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

    For Research Use Only. Not for Use in Diagnostic Procedures.
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