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Malic Enzyme 3 Antibody #52016

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Inquiry Info. # 52016

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 67
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Malic Enzyme 3 Antibody recognizes endogenous levels of total malic enzyme 3 protein. This antibody does not cross-react with malic enzyme 1 and malic enzyme 2 proteins.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala390 of human malic enzyme 3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Malic enzymes catalyze oxidative decarboxylation of malate to pyruvate (1). The malic enzyme family in mammalian cells includes the cytosolic malic enzyme 1 (ME1) and two mitochondrial malic enzymes (ME2 and ME3) (1, 2). ME1 and ME2 are critical for tumor cell growth and their expression is repressed by tumor suppressor p53 (2). Reduced expression of ME1 and ME2 reciprocally increases the levels and activation of p53, promoting p53-mediated senescence (2). Research studies show ME3 is essential for the survival of pancreatic ductal adenocarcinoma following genomic deletion of ME2 (3). Deletion of ME3 is lethal to ME2-null cancer cells, which has been suggested to provide a potential therapeutic opportunity using collateral lethality (3, 4).

    Alternate Names

    FLJ34862; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); Malic enzyme 3; malic enzyme 3, NADP(+)-dependent, mitochondrial; malic enzyme, NADP+-dependent, mitochondrial; MAON; ME3; mitochondrial NADP(+)-dependent malic enzyme 3; NADP-dependent malic enzyme, mitochondrial; NADP-ME; pyruvic-malic carboxylase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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