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9926
MAPK Family Antibody Sampler Kit

MAPK Family Antibody Sampler Kit #9926

Western Blotting Image 1

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from 293 and SK-N-MC cells, untreated or UV-treated (40 J/m2), using Phospho-SAPK/JNK Antibody #9251 (upper) or SAPK/JNK Antibody (lower).

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using p38 MAPK (D13E1) XP® Rabbit mAb.

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Western Blotting Image 4

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 5

Western blot analysis of extracts from Hek 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® p44/42 MAPK (Erk1/2) siRNA (+), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 and α-Tubulin (11H10) Rabbit mAb #2125. The p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb confirms silencing of p44/42 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p44/42 MAPK (Erk1/2) siRNA.

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IHC-P (paraffin) Image 6

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb.

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IHC-P (paraffin) Image 7

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb.

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IHC-P (paraffin) Image 8

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb.

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb in the presence of control peptide (left) or #1240 p44/42 MAPK (Erk1/2) Blocking Peptide (#4695 Specific) (right).

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Flow Cytometry Image 12

Flow cytometric analysis of HeLa cells using p38 MAPK (D13E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

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Flow Cytometry Image 13

Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb compared to a nonspecific negative control antibody (red).

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IF-IC Image 14

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with UV (100 mJ/cm2 with 30 min recovery; right), using p38 MAPK (D13E1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

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IF-IC Image 15

Confocal immunofluorescent analysis of NIH/3T3 cells, treated with either U0126 (MEK1/2 Inhibitor) #9903 (left) or PDGF (right), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb 4695 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R Hm Mk Mi Dm Z B Dg Pg Ce 42, 44 Rabbit IgG
SAPK/JNK Antibody 9252 20 µl
  • WB
H M R Hm Mk Z B Sc 46, 54 Rabbit 
p38 MAPK (D13E1) XP® Rabbit mAb 8690 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Hm Mk B Pg 40 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The MAPK Family Antibody Sampler Kit provides an economical means of evaluating total levels of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibody to perform two western blot experiments.

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.

p44/42 MAP Kinase (137F5) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. p38 MAPK (D13E1) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human p38 protein. SAPK/JNK Antibody is produced by immunizing animals with a recombinant human JNK2 fusion protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).

  1. Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
  2. Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
  3. Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
  4. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
  5. Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.
Entrez-Gene Id
5595 , 5594 , 1432 , 5600 , 6300 , 5599
Swiss-Prot Acc.
P27361 , P28482 , Q16539 , Q15759 , P53778 , P45983
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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