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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb 4695 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk Mi Dm Z B Dg Pg Ce 42, 44 Rabbit IgG
SAPK/JNK Antibody 9252 40 µl
Western Blotting
H M R Hm Mk Z B Sc 46, 54 Rabbit 
p38 MAPK (D13E1) XP® Rabbit mAb 8690 40 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Hm Mk B Pg 40 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The MAPK Family Antibody Sampler Kit provides an economical means of evaluating total levels of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibody to perform four western blot experiments.


Specificity / Sensitivity

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.


Source / Purification

p44/42 MAP Kinase (137F5) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. p38 MAPK (D13E1) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human p38 protein. SAPK/JNK Antibody is produced by immunizing animals with a recombinant human JNK2 fusion protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).


1.  Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.

2.  Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.

3.  Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.

4.  Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

5.  Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.


Entrez-Gene Id 5595 , 5594 , 1432 , 5600 , 6300 , 5599
Swiss-Prot Acc. P27361 , P28482 , Q16539 , Q15759 , P53778 , P45983


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

9926
MAPK Family Antibody Sampler Kit