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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MAST1 (E4K5G) Rabbit mAb #38959

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  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 210
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MAST1 (E4K5G) Rabbit mAb recognizes endogenous levels of total MAST1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys764 of human MAST1 protein.

    Background

    MAST1 is a member of the microtubule-associated serine/threonine kinase (MAST) family of protein kinases. It possesses a serine/threonine kinase domain and a PDZ domain, the latter being important for its function as a scaffold molecule linking the dystrophin and utrophin complex with microfilaments (1,2). Expression of the gene encoding MAST1 is upregulated following glucocorticoid receptor activation, while MAST1 protein expression levels can be maintained by complex formation with HSP90B, protecting MAST1 from degradation (3,4). MAST1 gene rearrangements have been found in breast cancers, where MAST1 was reported to promote cell proliferation and tumor growth (5). MAST1 was also identified as a critical factor involved in the development of cisplatin resistance, via binding and phosphorylation of MEK1 that led to inhibition of apoptosis (6). MAST1 also plays an important role in neuron development and function (7). Numerous mutations in the gene encoding MAST1 are reported to associate with neural disorders, including mega-corpus-callosum syndrome, congenital bilateral perisylvian syndrome (CBPS), and intellectual disability (ID) (8-10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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