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MBOAT7 (F6A3Y) Rabbit mAb #99001

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MBOAT7 (F6A3Y) Rabbit mAb recognizes endogenous levels of total MBOAT7 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro466 of human MBOAT7 protein.

    Background

    Membrane-bound O-acyltransferase domain-containing protein 7 (MBOAT7), also called lysophosphatidylinositol acyltransferase 1 (LPIAT1), is an enzyme involved in phospholipid metabolism by facilitating the addition of phosphatidylinositol onto arachidonic acid (1,2). MBOAT7 can promote ferroptosis, an iron-dependent form of cell death characterized by peroxidation of polyunsaturated fatty acids (3). MBOAT7 plays an important role in diseases, including liver disease, cancer, and neurodegeneration. Loss of MBOAT7 in mice results in atrophy of the cerebral cortex and hippocampus (4). MBOAT7-driven lipid remodeling promotes the progression of clear cell renal carcinoma (5). In non-small cell lung cancer, MBOAT7 levels are elevated and support prostaglandin synthesis, cell proliferation, and anchorage-independent growth (6). Genome-wide association studies (GWAS) have identified a link between single-nucleotide polymorphisms (SNPs) in the MBOAT7 gene locus with advanced liver disease (7-10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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