MED12 (D9K5J) Rabbit mAb #14360
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 240 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
MED12 is part of the CDK8 submodule of the mediator complex and is required for the stable interaction of this module with the rest of the mediator complex (1,9). The CDK8 module has been shown to be both a negative and positive regulator of transcription, depending on the gene context. The CDK8 module may repress transcription by inhibiting the ability of mediator to recruit RNAPII (10). In addition, the MED12 subunit can recruit the methyltransferase G9a to methylate histone H3K9 to repress a subset of neuronal genes in non-neuronal cells (11). MED12 and the CDK8 module can also positively regulate transcription of Wnt-responsive genes through its interaction with β-catenin, and p53-regulated genes upon UV-induced DNA damage (1,9,12).
- Taatjes, D.J. (2010) Trends Biochem Sci 35, 315-22.
- Kornberg, R.D. (2005) Trends Biochem Sci 30, 235-9.
- Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
- Malik, S. et al. (2002) Mol Cell Biol 22, 5626-37.
- Wang, G. et al. (2005) Mol Cell 17, 683-94.
- Kagey, M.H. et al. (2010) Nature 467, 430-5.
- Park, S.W. et al. (2005) Mol Cell 19, 643-53.
- Chen, Z. et al. (2011) EMBO J 30, 2405-19.
- Kim, S. et al. (2006) J Biol Chem 281, 14066-75.
- Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51.
- Ding, N. et al. (2008) Mol Cell 31, 347-59.
- Galbraith, M.D. et al. (2010) Transcription 1, 4-12.
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