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Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

Methyl-Histone H3 (Lys36) Antibody Sampler Kit #46599

    Product Information

    Product Description

    The Methyl-Histone H3 (Lys36) Antibody Sampler Kit provides an economical means of detecting levels of mono-, di-, and tri-methyl histone H3 Lys36 using methyl-specific and control histone H3 antibodies. The kit contains enough primary antibodies to perform at least two western blot experiments.

    Specificity / Sensitivity

    Each antibody in the Methyl-Histone H3 (Lys36) Antibody Sampler Kit detects endogenous levels of its target protein. Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb detects endogenous levels of histone H3 only when tri-methylated on Lys36. Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb detects endogenous levels of histone H3, only when di-methylated on Lys36. Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb recognizes endogenous levels of histone H3 only when mono-methylated at Lys36. Histone H3 (D1H2) XP® Rabbit mAb detects endogenous levels of total Histone H3 protein, including isoforms H3.1, H3.2, H3.3, and the variant histone CENP-A. This antibody does not cross-react with other core histones.

    Source / Purification

    Monoclonal methyl-histone H3 Lys36 antibodies are produced by immunizing rabbits with synthetic peptides corresponding to the amino terminus of histone H3 in which Lys36 is mono-, di-, or tri-methylated. The control histone H3 monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human histone H3 protein.

    Background

    The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases, such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1, has shown that methylation is a reversible epigenetic marker (9).

    Methylation of histone H3 Lys36 is associated with transcriptionally active genes. Tri- and di-methyl-histone H3 Lys36 levels are high in the bodies of active genes, where these marks function to repress intragenic transcription initiation and regulate mRNA splicing. Mono-methyl-histone H3 Lys36 levels are high in the bodies of both active and inactive genes.
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    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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