MHC Class I Antigen Processing and Presentation Antibody Sampler Kit #36064
Product Information
Kit Usage Information
Protocols
- 2679: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*
- 7074: Western Blotting
- 10405: Western Blotting, Immunoprecipitation (Agarose)
- 12238: Western Blotting, Immunofluorescence*, Flow
- 12851: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 13635: Western Blotting
- 25657: Western Blotting, Immunoprecipitation (Agarose)
- 43124: Western Blotting
- 49671: Western Blotting, Immunoprecipitation (Agarose)
- 79769: Western Blotting, Immunohistochemistry (Paraffin)
Product Description
Specificity / Sensitivity
Each antibody in the MHC Class I Antigen Processing and Presentation Antibody Sampler Kit detects endogenous levels of its target protein. Calreticulin (D3E6) XP® Rabbit mAb recognizes endogenous levels of total calreticulin protein. Ubiquitin (E4I2J) Rabbit mAb recognizes endogenous levels of free ubiquitin and polyubiquitinated proteins. This antibody is able to detect free ubiquitin, linear polyubiquitin (M1-linked), and homotypic polyubiquitin chains consisting of K6, K11, K27, K29, K33, K48 and K63 linkages. HLA-G (E8N9C) XP® Rabbit mAb recognizes endogenous levels of total HLA-G protein. Calnexin (C5C9) Rabbit mAb detects endogenous levels of total calnexin protein. TAP2 (E8G5I) Rabbit mAb recognizes endogenous levels of total TAP2 protein. This antibody does not cross-react with TAP1 protein. PSMB8/LMP7 (D1K7X) Rabbit mAb recognizes endogenous levels of total PSMB8/LMP7 protein. This antibody recognizes both 28 kDa precursor and 23 kDa mature forms of PSMB8/LMP7 and does not cross-react with PSMB5 protein. This antibody recognizes proteins of unknown origin in the 80-100 kDa range. TAP1 (E4T4F) Rabbit mAb recognizes endogenous levels of total TAP1 protein. This antibody does not cross-react with TAP2 protein. β2-microglobulin (D8P1H) Rabbit mAb recognizes endogenous levels of total β2-microglobulin protein. IFNGR1 (E444) Antibody recognizes endogenous levels of total IFNGR1 protein.
Source / Purification
Monoclonal antibodies are produced by immunizing animals either with a recombinant protein specific to the carboxy terminus of human TAP1 protein or a synthetic peptide corresponding to residues near the amino terminus of human calreticulin protein, the carboxy terminus of human PSMB8/LMP7 protein, Gly35 of human ubiquitin protein, Leu102 of human HLA-G protein, Val57 of human β2-microglobulin protein, Val485 of human TAP2 protein, or Pro52 of human calnexin protein. Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu444 of human IFNGR1 protein. Polyclonal antibody is purified by protein A and peptide affinity chromatography.
Background
The predominant function of class I MHC/β2-microglobulin dimers, which are expressed on the surface of most nucleated cell types, is to modulate the adaptive immune response by presenting proteolytic peptide fragments from cytosolic proteins to cytotoxic CD8+ T cells. In order for self and nonself peptides to be presented by MHC class I molecules, the peptide fragments must first be derived from polyubiquitinated proteins that undergo degradation via the ubiquitin-proteasome system. In the context of inflammatory processes, the enzymatic core of the proteasome can be shaped by IFNγ signaling to contain subunits, such as PSMB8/LMP7, which enhance the presentation of antigenic peptides by antigen presenting cells (1). The resulting cytosolic peptide fragments generated through ubiquitin-dependent proteasomal degradation are then transported into the ER lumen via the peptide transporters, TAP1 and TAP2, where the activity of multiple chaperone proteins, such as calnexin and calreticulin, facilitate loading onto class I MHC/β2-microglobulin dimers for transport to the Golgi and eventually, the cell surface (2-6). Defects in the expression of multiple components of the class I antigen presenting machinery have been observed in both solid and liquid tumors, which serves as a mechanism of tumor-immune evasion (7).
- Ferrington, D.A. and Gregerson, D.S. (2012) Prog Mol Biol Transl Sci 109, 75-112.
- Antoniou, A.N. et al. (2003) Curr Opin Immunol 15, 75-81.
- Jensen, P.E. (2007) Nat Immunol 8, 1041-8.
- Kloetzel, P.M. (2001) Nat Rev Mol Cell Biol 2, 179-87.
- Sant, A. and Yewdell, J. (2003) Curr Opin Immunol 15, 66-8.
- Yewdell, J.W. (2005) Immunol Rev 207, 8-18.
- Seliger, B. (2008) Cancer Immunol Immunother 57, 1719-26.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.