Render Target: STATIC
Render Timestamp: 2024-11-11T10:11:33.751Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:59:53.317
Product last modified at: 2024-09-30T08:01:53.081Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MHC Class I (E6L8F) Rabbit mAb #35923

Filter:
  • WB
  • IP
  • F

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 40-50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MHC Class I (E6L8F) Rabbit mAb recognizes endogenous levels of total MHC Class I protein. An unknown background band is detected at 100 kDa in some cell lines.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu254 of mouse H2-D1 protein.

    Background

    The human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8+ T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4+ T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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