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43816
MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free) #43816

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  1. WB
  2. IHC
Western Blotting Image 1: MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using MHC Class II (LGII-612.14) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using MHC Class II (LGII-612.14) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using MHC Class II (LGII-612.14) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using MHC Class II (LGII-612.14) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using MHC Class II (LGII-612.14) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MHC Class II (LGII-612.14) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Raji cell pellet (left, positive) or Jurkat cell pellet (right, negative) using MHC Class II (LGII-612.14) Mouse mAb. Data were generated using the standard formulation of this product.
To Purchase # 43816
Cat. # Size Qty. Price
43816SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 25-35, 50-65
Source/Isotype Mouse IgG1

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #68258. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #68258

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MHC Class II (LGII-612.14) Mouse mAb (BSA and Azide Free) exhibits strong reactivity with HLA-DRB and weak reactivity with HLA-DPB in cell lines transfected with constructs expressing Myc/DDK-tagged HLA-DRB and HLA-DPB, respectively. Reactivity is not observed with HLA-DMB, HLA-DOB, and HLA-DQB in cell lines transfected with constructs expressing Myc/DDK-tagged HLA-DMB, HLA-DOB, and HLA-DQB.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with cultured human B lymphoid cells treated with IFN-gamma.

Background

Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).
In the literature, this clone is reported to react with HLA-DR, HLA-DP, and HLA-DQ (4).
  1. Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
  2. Cresswell, P. (1994) Annu Rev Immunol 12, 259-93.
  3. Karp, D.R. et al. (1990) J Exp Med 171, 615-28.
  4. Temponi, M. et al. (1993) J Immunol Methods 161, 239-56.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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