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MIA40/CHCHD4 (F8J4L) Rabbit mAb

MIA40/CHCHD4 (F8J4L) Rabbit mAb #48362

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	20
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MIA40/CHCHD4 (F8J4L) Rabbit mAb recognizes endogenous levels of total MIA40/CHCHD4 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala128 of human MIA40/CHCHD4 protein.

Background

Mitochondrial intermembrane space import and assembly protein 40 (MIA40), also known as coiled-coil-helix-coiled-coil-helix domain-containing protein 4 (CHCHD4), is a chaperone and oxidoreductase in the mitochondrial intermembrane space (IMS). It is an import receptor for various intermembrane space proteins and has a key role in their oxidative folding (1,2). Studies show that the IMS protein AIFM1 mediates the import of MIA40/CHCHD4. Furthermore, AIFM1 forms a stable complex with MIA40/CHCHD4. This complex is crucial for the import of MIA40/CHCHD4 substrates into the IMS (1). In addition, the mitochondrial inner-membrane-associated protein C9orf72 is imported into the IMS via AIFM1-MIA40/CHCHD4 pathway. C9orf72 then stabilizes the assembly of mitochondrial complex I (3).

Database Links

UniProt ID: Q8N4Q1

Entrez-Gene Id: 131474

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For Research Use Only. Not For Use In Diagnostic Procedures.

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