Render Target: STATIC
Render Timestamp: 2024-12-02T11:04:42.958Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:57:40.713
Product last modified at: 2024-11-22T11:15:09.516Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MICA/B (E9N7G) Rabbit mAb #64899

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60, 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MICA/B (E9N7G) Rabbit mAb recognizes endogenous levels of total MICA and MICB proteins.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus (extracellular region) of human MICB protein.

    Background

    Natural killer group 2D (NKG2D) is an activating receptor expressed on NK and some T cells. It recognizes a variety of ligands that belong to the non-classical MHC class I family, including MICA, MICB, ULBP1–6 in human and Mult1, H60 (a, b, and c), and Rae-1 (α-ε) in mice. These ligand proteins are not expressed in most tissues under normal conditions, but are expressed in cells under stress, such as viral infection and malignant transformation. Engagement of NKG2D by these ligands activates cytolytic activity of NK and T cells against target cells, providing immunosurveillance. Some of these ligands are polymorphic. There are more than 100 different alleles for MICA, 40 for MICB, and around 20 for ULBPs (1-4). In addition, some of these ligands can be proteolytically cleaved from the cell surface to become soluble forms. The interaction of soluble ligands with NKG2D results in endocytosis and degradation of ligand-receptor complexes, therefore suppressing NKG2D-mediated immunosurveillance (5,6). However, recent data also demonstrated that soluble ligands can promote NK cell activation and tumor rejection (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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