Miz-1 Antibody

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WB

Inquiry Info. # 9044

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Product Specifications

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	95
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Miz-1 Antibody recognizes endogenous levels of total Miz-1 protein. This antibody cross-reacts with a 60 kDa protein of unknown origin in some cell lines.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro158 of human Miz-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Miz-1 (Zbtb17) is a poxvirus and zinc finger (POZ) transcription factor with an amino-terminal BTB/POZ domain and 13 carboxy-terminal zinc finger domains. Miz-1 plays a key role in cell cycle control through activation of the cyclin-dependent kinase inhibitors p15, INK4B, and p21 Waf1/Cip1 (1-4). The transcriptional activity of Miz-1 is repressed through direct interaction with Myc (1-4). In the presence of DNA damage, Myc is recruited to the p21 Waf1/Cip1 promoter by Miz-1 and blocks p53-mediated induction of p21 Waf1/Cip1, ultimately resulting in p53-mediated apoptosis rather than cell cycle arrest (4). Miz-1 also plays a role during lymphocyte development. In developing B and T cells, Miz-1 represses suppressor of cytokine signaling 1 (SOCS1) expression, which enables signaling through the IL-7 receptor and upregulation of the pro-survival protein Bcl-2 (5,6).

Alternate Names

Miz-1; MIZ1; Myc-interacting zinc finger protein 1; Myc-interacting Zn finger protein-1; pHZ-67; ZBT17; ZBTB17; zinc finger and BTB domain containing 17; Zinc finger and BTB domain-containing protein 17; Zinc finger protein 151; zinc finger protein 151 (pHZ-67); Zinc finger protein 60; ZNF151; ZNF60

Database Links

UniProt ID: Q13105

Entrez-Gene Id: 7709

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Miz-1 Antibody #9044