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Primary Antibodies /
MLK3 Antibody

MLK3 Antibody #2812

Inquiry Info. # 2812

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	120
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MLK3 Antibody detects endogenous levels of MLK3. This antibody may cross-react with MLK1 and MLK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr277 and Ser281 of human MLK3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Mixed lineage kinase 3 (MLK3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. CRIB triggers the dimerization of MLK3 via its tandem leucine zippers, followed by the intramolecular phosphorylation and subsequent activation of MLK3 (1). Autophosphorylation of Thr277 and Ser281 is essential for MLK3 kinase activity (2). Ser281 is also phosphorylated by HPK in an in vitro kinase assay (2). MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation (3). MLK3 also functions as an IκB kinase and mediates the activation of the transcriptional factor NF-κB stimulated by CD3/CD28, suggesting a role for MLK3 in immune and inflammatory responses (4).

Alternate Names

M3K11; MAP3K11; MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed-lineage kinase 3; MLK-3; MLK3; protein-tyrosine kinase PTK1; PTK1; SH3 domain-containing proline-rich kinase; SPRK; Src-homology 3 domain-containing proline-rich kinase

Database Links

UniProt ID: Q16584

Entrez-Gene Id: 4296

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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