MMP-1 (E9S9N) Rabbit mAb #54376
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #87303.
Protocol
Specificity / Sensitivity
MMP-1 (E9S9N) Rabbit mAb recognizes endogenous levels of total MMP-1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val267 of human MMP-1 protein.
Background
The matrix metalloproteinase (MMP) family of proteases is a group of zinc-dependent enzymes that target extracellular proteins, including growth factors, cell surface receptors, adhesion molecules, matrix structural proteins, and other proteases (1,2). Within this family, MMP-1, MMP-8, and MMP-13 have been characterized as a collagenase subfamily of MMPs targeting fibrillar collagen (collagen type I, II, and III) for degradation. In addition to collagen, MMP-1 also has activity toward a broad array of other ECM proteins, such as fibronectin, gelatin, aggrecan (etc.), as well as growth factors, chemokines, and cytokines (3). MMP-1 is widely involved in tissue remodeling during wound healing, tumor growth, invasion and metastasis, and arthritis (4-6).
- Löffek, S. et al. (2011) Eur Respir J 38, 191-208.
- Nagase, H. et al. (2006) Cardiovasc Res 69, 562-73.
- Foley, C.J. and Kuliopulos, A. (2014) J Cell Physiol 229, 1875-80.
- Ala-aho, R. and Kähäri, V.M. (2005) Biochimie 87, 273-86.
- Rohani, M.G. and Parks, W.C. (2015) Matrix Biol 44-46, 113-21.
- Burrage, P.S. et al. (2006) Front Biosci 11, 529-43.
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