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MMS19 (D5J8J) Rabbit mAb #90637

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  • IP

Inquiry Info. # 90637

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MMS19 (D5J8J) Rabbit mAb recognizes endogenous levels of total MMS19 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu892 of human MMS19 protein.

    Background

    Iron-sulfur (Fe-S) clusters (ISC) are cofactors for many proteins that display a wide range of biological functions, such as DNA maintenance, transcription, translation, cellular metabolism, electron transport, and oxidative phosphorylation (1). While structurally simple, the synthesis and insertion of ISC into Fe-S proteins are complex processes that involve many different proteins. MMS19 (methyl-methanesulfonate sensitivity 19) is a key component of the cytosolic Fe-S protein assembly complex, acting as an adaptor for a subset of Fe-S proteins including XPD and FANCJ, therefore playing an important role in DNA repair and transcription (2-4). MMS19 may also function as a coactivator for the estrogen receptor (5). In addition it has been reported that MMS19 is in a complex that is localized to the mitotic spindle and is required for proper chromosome segregation during mitosis (6).

    Alternate Names

    CIAO4; FLJ34167; FLJ95146; hMMS19; homolog of yeast MMS19; MET18; MET18 homolog; MGC99604; MMS19; MMS19 (MET18 S. cerevisiae)-like; MMS19 cytosolic iron-sulfur assembly component; MMS19 homolog, cytosolic iron-sulfur assembly component; MMS19 nucleotide excision repair homolog; MMS19 nucleotide excision repair homolog (S. cerevisiae); MMS19 nucleotide excision repair protein homolog; MMS19-like (MET18 homolog, S. cerevisiae); MMS19-like protein; MMS19L

    For Research Use Only. Not for Use in Diagnostic Procedures.
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