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37495
Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit

Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit #37495

Western Blotting Image 1

Western blot analysis of extracts from mouse spleen, EL4 cells, and Raji cells using CD3ε (D4V8L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-Leica® Bond™ Image 2

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD3ε (D4V8L) Rabbit mAb performed on the Leica® BOND™ Rx.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines and tissues using CD4 (D7D2Z) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-Leica® Bond™ Image 4

Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using CD4 (D7D2Z) Rabbit mAb performed on the Leica® BOND™ Rx.

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Western Blotting Image 5

Western blot analysis of extracts from mouse CD8+ T cells, mouse spleen, EL4 cells, and Raw264.7 cells using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-Leica® Bond™ Image 6

Immunohistochemical analysis of paraffin-embedded mouse lung using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) performed on the Leica® BOND™ Rx.

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IHC-Leica® Bond™ Image 7

Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using FoxP3 (D6O8R) Rabbit mAb performed on the Leica® BOND™ Rx.

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IF-F Image 8

Immunofluorescent analysis of mouse spleen using FoxP3 (D6O8R) Rabbit mAb (red) and a CD4 antibody (green). Blue = DRAQ5® #4084 (fluorescent DNA dye).

Note: FoxP3 (D6O8R) Rabbit mAb has been validated on freshly-prepared frozen tissue sections that were fixed for 15 minutes in 4% formaldehyde. Over-fixation diminishes the performance of this antibody.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded mouse liver using F4/80 (D2S9R) XP® Rabbit mAb.

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Western Blotting Image 10

Western blot analysis of extracts from various cell lines using F4/80 (D2S9R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 11

Western blot analysis of extracts from various cell lines using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD19 protein is not detected in either U-937 cells or Raw 264.7 cells.

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IHC-Leica® Bond™ Image 12

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using CD19 (D4V4B) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

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Western Blotting Image 13

Western blot analysis of extracts from Raw 264.7, C2C12, and 3T3 cells using CD11c (D1V9Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-Leica® Bond™ Image 14

Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using CD11c (D1V9Y) Rabbit mAb performed on the Leica® Bond™ Rx.

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IHC-Leica® Bond™ Image 15

Immunohistochemical analysis of paraffin-embedded human colon adenocarinoma using Granzyme B (D6E9W) Rabbit mAb performed on the Leica® BOND™ Rx.

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Western Blotting Image 16

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD3ε (D4V8L) Rabbit mAb.

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IHC-P (paraffin) Image 18

Immunohistochemical analysis of paraffin-embedded mouse colon using CD4 (D7D2Z) Rabbit mAb.

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IHC-P (paraffin) Image 19

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific).

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IHC-P (paraffin) Image 20

Immunohistochemical analysis of paraffin-embedded mouse thymus using FoxP3 (D6O8R) Rabbit mAb.

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IHC-P (paraffin) Image 21

Immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) XP® Rabbit mAb.

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Western Blotting Image 22

Western blot analysis of extracts from Daudi cells, untreated (-) or treated with PNGase F (+), using CD19 (D4V4B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-P (paraffin) Image 23

Immunohistochemical analysis of paraffin-embedded mouse kidney using CD11c (D1V9Y) Rabbit mAb.

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IHC-Leica® Bond™ Image 24

Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using Granzyme B (D6E9W) Rabbit mAb performed on the Leica® BOND™ Rx.

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IHC-P (paraffin) Image 25

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD3ε (D4V8L) Rabbit mAb.

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IHC-P (paraffin) Image 26

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD4 (D7D2Z) Rabbit mAb.

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IHC-P (paraffin) Image 27

Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific).

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IHC-P (paraffin) Image 28

Immunohistochemical analysis of paraffin-embedded mouse spleen using FoxP3 (D6O8R) Rabbit mAb.

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IHC-P (paraffin) Image 29

Immunohistochemical analysis of paraffin-embedded mouse small intestine using F4/80 (D2S9R) XP® Rabbit mAb.

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IHC-P (paraffin) Image 30

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD19 (D4V4B) XP® Rabbit mAb.

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IHC-P (paraffin) Image 31

Immunohistochemical analysis of paraffin-embedded mouse liver using CD11c (D1V9Y) Rabbit mAb.

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IHC-P (paraffin) Image 32

Immunohistochemical analysis of paraffin-embedded human papillary carcinoma of the breast using Granzyme B (D6E9W) Rabbit mAb.

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IHC-P (paraffin) Image 33

Immunohistochemical analysis of paraffin-embedded mouse lung using CD3ε (D4V8L) Rabbit mAb.

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IHC-P (paraffin) Image 34

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD4 (D7D2Z) Rabbit mAb.

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IHC-P (paraffin) Image 35

Immunohistochemical analysis of paraffin-embedded mouse small intestine using FoxP3 (D6O8R) Rabbit mAb.

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IHC-P (paraffin) Image 36

Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or Neuro-2A cell pellet (right, negative) using F4/80 (D2S9R) XP® Rabbit mAb.

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IHC-P (paraffin) Image 37

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD19 (D4V4B) XP® Rabbit mAb.

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IHC-P (paraffin) Image 38

Immunohistochemical analysis of paraffin-embedded mouse lung using CD11c (D1V9Y) Rabbit mAb.

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IHC-P (paraffin) Image 39

Immunohistochemical analysis of paraffin-embedded mouse 4T1 mammary tumor using Granzyme B (D6E9W) Rabbit mAb.

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IHC-P (paraffin) Image 40

Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using CD3ε (D4V8L) rabbit mAb (red), F4/80 (D2S9R) XP® rabbit mAb #70076 (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan).

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IHC-P (paraffin) Image 41

Immunohistochemical analysis of paraffin-embedded rat lung using CD4 (D7D2Z) Rabbit mAb.

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IHC-P (paraffin) Image 42

Immunohistochemical analysis of paraffin-embedded mouse lung using FoxP3 (D6O8R) Rabbit mAb.

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Flow Cytometry Image 43

Flow cytometric analysis of murine spleen lymphocytes using a CD25 antibody and FoxP3 (D6O8R) Rabbit mAb. Anti-rabbit IgG (H+L), (F(ab')2 ) Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on a gated CD4+ T cell population.

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IHC-P (paraffin) Image 44

Immunohistochemical analysis of paraffin-embedded human lymphoma using CD19 (D4V4B) XP® Rabbit mAb.

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IHC-P (paraffin) Image 45

Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or C2C12 cell pellet (right, negative) using CD11c (D1V9Y) Rabbit mAb.

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IHC-P (paraffin) Image 46

Immunohistochemical analysis of paraffin-embedded KARPAS-299 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using Granzyme B (D6E9W) Rabbit mAb. KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

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IHC-P (paraffin) Image 47

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Granzyme B (D6E9W) Rabbit mAb.

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IHC-P (paraffin) Image 48

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD19 (D4V4B) XP® Rabbit mAb.

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IHC-P (paraffin) Image 49

Immunohistochemical analysis of paraffin-embedded mouse spleen using CD11c (D1V9Y) Rabbit mAb.

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Western Blotting Image 50

Western blot analysis of extracts from various human cells using Granzyme B (D6E9W) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). CD8+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (20 ng/ml). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

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IHC-P (paraffin) Image 51

Immunohistochemical analysis of paraffin-embedded human tonsil using CD19 (D4V4B) XP® Rabbit mAb.

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IF-IC Image 52

Confocal immunofluorescent analysis of Raw 264.7 cells (left, positive) and 3T3 cells (right, negative) using CD11c (D1V9Y) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

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Western Blotting Image 53

Western blot analysis of extracts from CTLL-2, mouse CD8+ T, and C2C12 cells using Granzyme B (D6E9W) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). CD8+ T cells were purified from mouse spleens and stimulated for 9 days using beads coated with mouse CD3 and CD28 antibodies in the presence of Mouse Interleukin-2 (mIL-2) #5201 (20 ng/ml).

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IHC-P (paraffin) Image 54

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using Granzyme B (D6E9W) Rabbit mAb.

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IF-F Image 55

Confocal immunofluorescent analysis of mouse spleen (left) and mouse kidney (right) using CD11c (D1V9Y) Rabbit mAb. Nuclei were labeled with ProLong® Gold Antifade reagent with DAPI #8961 (blue).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CD3ε (D4V8L) Rabbit mAb 99940 20 µl
  • WB
  • IHC
M 22 Rabbit IgG
CD4 (D7D2Z) Rabbit mAb 25229 20 µl
  • WB
  • IHC
M R 55 Rabbit IgG
CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) 98941 20 µl
  • WB
  • IHC
M 35-42 Rabbit IgG
FoxP3 (D6O8R) Rabbit mAb 12653 20 µl
  • IHC
  • IF
  • F
M Rabbit IgG
F4/80 (D2S9R) XP® Rabbit mAb 70076 20 µl
  • WB
  • IP
  • IHC
M 65-250 Rabbit IgG
CD19 (D4V4B) XP® Rabbit mAb 90176 20 µl
  • WB
  • IP
  • IHC
H M 95 Rabbit IgG
CD11c (D1V9Y) Rabbit mAb 97585 20 µl
  • WB
  • IHC
  • IF
M 145 Rabbit IgG
Granzyme B (D6E9W) Rabbit mAb 46890 20 µl
  • WB
  • IHC
H M 30 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit provides an economical means of detecting the accumulation of immune cell types in formalin-fixed, paraffin-embedded tissue samples.

Each antibody included in the Mouse Immune Cell Phenotyping IHC Antibody Sampler Kit recognizes endogenous levels of its target mouse protein. Non-specific staining has been observed in mouse pancreas with CD3ε (D4V8L) Rabbit mAb, in mouse kidney and liver with CD4 (D7D2Z) Rabbit mAb, and in the sweat glands with Granzyme B (D6E9W) Rabbit mAb.

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val31 of mouse CD3ε protein, Asp42 of mouse CD8α protein, Ala232 of mouse CD4 protein, Pro44 of mouse FoxP3 protein, Leu427 of human CD19 protein, Ala1153 of mouse CD11c protein, or with recombinant proteins specific to human Granzyme B protein or mouse F4/80 protein.

Cluster of Differentiation 3 (CD3) is a multiunit protein complex expressed on the surface of T-cells that directly associates with the T-cell receptor (TCR). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Engagement of TCR complex with antigens presented in Major Histocompatibility Complexes (MHC) induces tyrosine phosphorylation in the immunoreceptor tyrosine-based activation motif (ITAM) of CD3 proteins. CD3 phosphorylation is required for downstream signaling through ZAP-70 and p85 subunit of PI-3 kinase, leading to T cell activation, proliferation, and effector functions (1). Cluster of Differentiation 8 (CD8) is a transmembrane glycoprotein expressed primarily on cytotoxic T cells, but has also been described on a subset of dendritic cells in mice (2,3). On T cells, CD8 is a co-receptor for the TCR, and these two distinct structures are required to recognize antigen bound to MHC Class I (2). Cluster of Differentiation 4 (CD4) is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the TCR, and these two distinct structures recognize antigen bound to MHC Class II. CD8 and CD4 co-receptors ensure specificity of the TCR–antigen interaction, prolong the contact between the T cell and the antigen presenting cell, and recruit the tyrosine kinase Lck, which is essential for T cell activation (2). Granzyme B is a serine protease expressed by CD8+ cytotoxic T lymphocytes and natural killer (NK) cells and is a key component of the immune response to pathogens and transformed cancer cells (4). Forkhead box P3 (FoxP3) is crucial for the development of T cells with immunosuppressive regulatory properties and is a well-established marker for T regulatory cells (Tregs) (5). CD19 is a co-receptor expressed on B cells that amplifies the signaling cascade initiated by the B cell receptor (BCR) to induce activation. It is a biomarker of B lymphocyte development, lymphoma diagnosis, and can be utilized as a target for leukemia immunotherapies (6,7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8). CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein highly expressed by dendritic cells, and has also been observed on activated NK cells, subsets of B and T cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (9,10).

  1. Kuhns, M.S. et al. (2006) Immunity 24, 133-139.
  2. Zamoyska, R. (1994) Immunity 1, 243-46.
  3. Shortman, K. and Heath, W.R. (2010) Immunol Rev 234, 18-31.
  4. Trapani, J.A. (2001) Genome Biol 2, REVIEWS3014.
  5. Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
  6. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
  7. Scheuermann, R.H. and Racila, E. (1995) Leuk Lymphoma 18, 385-97.
  8. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  9. Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
  10. Qualai, J. et al. (2016) PLoS One 11, e0154253.
Entrez-Gene Id
12478 , 12501 , 12504 , 12525 , 13733 , 20371 , 14939 , 16411
Swiss-Prot Acc.
P25918 , P22646 , P06332 , P01731 , Q61549 , Q99JB6 , P04187 , Q9QXH4
For Research Use Only. Not For Use In Diagnostic Procedures.

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