|CD4 (D7D2Z) Rabbit mAb 25229||20 µl||
||M R Hm||55||Rabbit IgG|
|CD8α (D4W2Z) XP® Rabbit mAb 98941||20 µl||
|FoxP3 (D6O8R) Rabbit mAb 12653||20 µl||
||M Mk||Rabbit IgG|
|F4/80 (D2S9R) XP® Rabbit mAb 70076||20 µl||
|CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb 90176||20 µl||
||H M Mk||95||Rabbit IgG|
|CD11c (D1V9Y) Rabbit mAb 97585||20 µl||
|Granzyme B (E5V2L) Rabbit mAb 44153||20 µl||
|CD3ε (E4T1B) XP® Rabbit mAb 78588||20 µl||
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu50 of mouse CD3ε protein, Asp42 of mouse CD8α protein, Ala232 of mouse CD4 protein, Pro44 of mouse FoxP3 protein, Leu427 of human CD19 protein, Ala1153 of mouse CD11c protein, or with a synthetic peptide corresponding to residues surrounding Ala197 of mouse Granzyme B protein or mouse F4/80 protein.
Cluster of Differentiation 3 (CD3) is a multiunit protein complex expressed on the surface of T-cells that directly associates with the T-cell receptor (TCR). CD3 is composed of four polypeptides: ζ, γ, ε and δ. Engagement of TCR complex with antigens presented in Major Histocompatibility Complexes (MHC) induces tyrosine phosphorylation in the immunoreceptor tyrosine-based activation motif (ITAM) of CD3 proteins. CD3 phosphorylation is required for downstream signaling through ZAP-70 and p85 subunit of PI-3 kinase, leading to T cell activation, proliferation, and effector functions (1). Cluster of Differentiation 8 (CD8) is a transmembrane glycoprotein expressed primarily on cytotoxic T cells, but has also been described on a subset of dendritic cells in mice (2,3). On T cells, CD8 is a co-receptor for the TCR, and these two distinct structures are required to recognize antigen bound to MHC Class I (2). Cluster of Differentiation 4 (CD4) is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the TCR, and these two distinct structures recognize antigen bound to MHC Class II. CD8 and CD4 co-receptors ensure specificity of the TCR–antigen interaction, prolong the contact between the T cell and the antigen presenting cell, and recruit the tyrosine kinase Lck, which is essential for T cell activation (2). Granzyme B is a serine protease expressed by CD8+ cytotoxic T lymphocytes and natural killer (NK) cells and is a key component of the immune response to pathogens and transformed cancer cells (4). Forkhead box P3 (FoxP3) is crucial for the development of T cells with immunosuppressive regulatory properties and is a well-established marker for T regulatory cells (Tregs) (5). CD19 is a co-receptor expressed on B cells that amplifies the signaling cascade initiated by the B cell receptor (BCR) to induce activation. It is a biomarker of B lymphocyte development, lymphoma diagnosis, and can be utilized as a target for leukemia immunotherapies (6,7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8). CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein highly expressed by dendritic cells, and has also been observed on activated NK cells, subsets of B and T cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (9,10).
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