Mouse Reactive Senescence Marker Antibody Sampler Kit #78551
Product Information
Kit Usage Information
Protocols
- 6893: Western Blotting, Immunohistochemistry (Paraffin)
- 7074: Western Blotting
- 9718: Western Blotting, Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence*, Flow Cytometry, BSA-free Methanol Permeabilization Protocol
- 11948: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow
- 12912: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow
- 17416: Western Blotting, Immunohistochemistry (Paraffin)
- 29271: Western Blotting, Immunoprecipitation (Magnetic)
- 37543: Western Blotting, Immunoprecipitation (Magnetic)
- 87809: Western Blotting, Immunoprecipitation (Agarose)
Product Description
Specificity / Sensitivity
Each antibody in the Mouse Reactive Senescence Marker Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at Ser139.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro67 of mouse p16 INK4A protein, Arg455 of human lamin B1 protein, Ala137 of human HMGB1 protein, Pro117 of human MMP-2 protein, a synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X protein, or recombinant proteins specific to human p21 Waf1/Cip1 protein or mouse TNF-α protein.
Background
Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors, and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2).
Because there is no single biomarker that can be used to definitively identify senescent cells, researchers must rely on a collection of biomarkers commonly associated with senescence (3). The Mouse Reactive Senescence Marker Antibody Sampler Kit provides a collection of antibodies to commonly used biomarkers of senescence-associated cell cycle arrest (p16 INK4A, p21 Waf1/Cip1), senescence-associated DNA damage (gamma-Histone H2A.X), and the SASP (HMGB1, IL-6, TNF-α, MMP2). The kit also includes an antibody to lamin B1, which is frequently reduced in senescent cells.
Limited Uses
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