Render Target: STATIC
Render Timestamp: 2024-12-10T10:58:22.769Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:57:52.180
Product last modified at: 2024-09-30T08:00:31.696Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MRCKα (E2S5I) Rabbit mAb #81681

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:3200 - 1:12800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MRCKα (E2S5I) Rabbit mAb recognizes endogenous levels of total MRCKα protein. Based on the sequence of the peptide antigen, this antibody is not expected to cross-react with MRCKβ or MRCKγ.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1728 of human MRCKα protein.

    Background

    CDC42BPA/MRCK (Myotonic dystrophy kinase-related CDC42-binding kinase) comprises a family of serine/threonine kinases that function as downstream effectors of the small GTPase, CDC42. Three MRCK family members have been identified: MRCKα, MRCKβ, and MRCKγ. MRCK family kinases have some substrates in common with the Rho-associated kinases, ROCK1 and ROCK2, including Myosin Light Chain 2 (MLC2). Signaling through both ROCK and MRCK kinase families regulates actin dynamics, cell migration, invasion, and metastasis (1,2). Simultaneous inhibition of both ROCK and MRCK kinases prevents migration and invasion of human cancer cell lines (3). Small molecule inhibitors targeting the MRCK family are being investigated as potential treatments for glioblastoma and skin cancer (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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