Render Target: STATIC
Render Timestamp: 2024-12-12T11:51:52.450Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-01 15:26:52.138
Product last modified at: 2024-08-20T18:15:08.721Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MTSS1 (D2H4L) XP® Rabbit mAb #93065

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 95-105
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:200
    Immunofluorescence (Immunocytochemistry) 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MTSS1 (D2H4L) XP® Rabbit mAb recognizes endogenous levels of total MTSS1 protein.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val650 of human MTSS1 protein.

    Background

    Metastasis suppressor 1 (MTSS1) is a multi-functional scaffold protein that was initially discovered using a differential display technique that identified proteins missing from bladder cancer cell lines (1,2). MTSS1 (also known as Missing in Metastasis or MIM) is a cytoskeletal remodeling protein that contains a C-terminal WH2 actin-binding motif (1,3). Presence of an IMD (IRSp53/MIM homology) domain allows MTSS1 to induce F-actin bundling and filopodia formation in cells (4). MTSS1 binds to and activates Rac, a protein known to promote the formation of filopodia and lamellipodia (5). The receptor tyrosine phosphatase δ (PTPRD) is associated with MTSS1 and is required for MTSS1-dependent cytoskeletal change (6,7). MTSS1 is a SHH responsive gene that can help regulate GLI-dependent transcriptional activity (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SignalStain is a registered trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.