Render Target: STATIC
Render Timestamp: 2025-02-12T11:03:23.687Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2025-01-14 23:01:18.814
Product last modified at: 2025-01-17T09:00:10.059Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MUC13 (E6Z1K) Rabbit mAb (BSA and Azide Free) #17339

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  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70-90,120-175
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #44454. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #44454

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    MUC13 (E6Z1K) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total MUC13 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MUC13 protein.

    Background

    Mucin proteins are glycoproteins known for providing protection and lubrication to the luminal epithelial tissues under normal physiologic conditions (1,2). There are three types of mucins based on their structure and location: transmembrane, secreted, and soluble. Mucins can function as oncogenes and exert the effect of tumor-promoting and/or tumor-suppressing factors (3). MUC13 (Mucin13) is a transmembrane mucin. The protein is expressed in the epithelium layer of large intestine, trachea, kidney, small intestine, stomach, and hematopoietic cells (4). There it regulates epithelial inflammation in response to inflammatory and infectious stimuli (5). MUC13 is aberrantly overexpressed in various cancer types, including gastric (6), pancreatic (7), hepatic (8), colonic (9), renal (10), etc. The protein promotes tumorigenesis, metastasis, and induces resistance to chemotherapy via activating multiple pathways, such as β-catenin signaling, Her2/EGFR signaling, and NF-kB signaling (8,9,11,12). The apparent molecular weight of MUC13 is varied from 70 to 90 kDa and 120 to 175 kDa in different cell lines, due to the different protein glycosylation status (13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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