Render Target: STATIC
Render Timestamp: 2024-11-05T09:41:34.174Z
Commit: 57f6e368eba1a427377652f2ad915d45d7f340a4
XML generation date: 2024-05-10 22:35:36.736
Product last modified at: 2024-10-31T13:15:15.374Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

MUC16 (IHC125) Mouse mAb #19017

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 200-270
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    IHC Leica Bond 1:800 - 1:3200
    Immunohistochemistry (Paraffin) 1:400 - 1:1600

    Storage

    Supplied in a Tris-based buffer with 1% BSA and less than 0.1% sodium azide. Stable for 24 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MUC16 (IHC125) Mouse mAb recognizes endogenous levels of total MUC16 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with full-length native MUC16 protein.

    Background

    Mucins are a family of macromolecules that line and protect the respiratory epithelium from microbes and pollutants in the local environment. Of the family members that are known to date, some are produced in a cell type and tissue-specific manner, suggesting distinct biological roles for members. Some members polymerize after secretion to form gel-like substances that coat the epithelial layer (1). MUC16 is a member of the mucin family, which are high molecular weight, O-glycosylated proteins that play an important role in forming mucous barriers (2) and are found on the apical surface of the epithelia. It contains an extracellular domain at its amino acid terminus, a large tandem repeat, and a transmembrane domain with a short cytoplasmic domain. MUC16 has been linked to lung cancer (3) and ovarian cancer (4). It provides protective, lubricating barriers against particles and infectious agents at mucosal surfaces (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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