MUC4 β (D4W9X) XP® Rabbit mAb #81692
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100-170 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:250 - 1:1000 |
Storage
Protocol
Specificity / Sensitivity
MUC4 β (D4W9X) XP® Rabbit mAb recognizes endogenous levels of total MUC4 protein (β subunit).
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MUC4 protein (β subunit).
Background
MUC4 is a membrane-bound mucin family member (1,2). It is a heterodimer of extracellular α subunits associated with a trans-membrane β subunit. There are 3 epidermal growth factor (EGF)-like domains in the extracellular region of the β subunit. These EGF-like domains can directly interact with ErbB2 to activate the downstream signaling pathways. MUC4 is expressed in the epithelial tissues of lung and gastrointestinal organs during development as well as in adult stage. It exerts a protective effect to these tissues against potential damage (3). Overexpression of MUC4 has been found in multiple types of cancers to promote cancer growth, survival, and metastasis (4-6). MUC4 has been proposed as a promising prognostic marker for cancer evaluation.
- Kasprzak, A. and Adamek, A. (2019) Int J Mol Sci 20, pii: E1288. doi: 10.3390/ijms20061288.
- Hanson, R.L. and Hollingsworth, M.A. (2016) Biomolecules 6, pii: E34. doi: 10.3390/biom6030034.
- Chaturvedi, P. et al. (2008) FASEB J 22, 966-81.
- Gautam, S.K. et al. (2017) Expert Opin Ther Targets 21, 657-69.
- Bafna, S. et al. (2010) Oncogene 29, 2893-904.
- Xia, P. et al. (2017) Oncotarget 8, 14147-57.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.