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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MuRF1 (F9X8V) Rabbit mAb #33973

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 41
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MuRF1 (F9X8V) Rabbit mAb recognizes endogenous levels of total MuRF1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human MuRF1 protein.

    Background

    Muscle-specific RING finger protein 1 (MuRF1) is an E3 ubiquitin ligase that promotes muscle cell protein degradation during muscle atrophy (1). In response to conditions that promote muscle atrophy, elevated skeletal muscle MuRF1 facilitates the ubiquitination and degradation of thick filament components (2). Cardiac muscle MuRF1 promotes cardiomyocyte troponin I degradation and inhibits PKCε-mediated cardiac hypertrophy (3,4). MuRF1 also regulates degradation of the large sarcomeric protein titin, the transcriptional regulator GMEB1, the SUMO conjugating enzyme UBC9, and the ubiquitin peptidase USP13 (5). Binding of FoxO1 and the glucocorticoid receptor to adjacent sites within the MuRF1 promoter region activates transcription of this E3 ubiquitin ligase (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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