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88757
Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free) #88757

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Myeloperoxidase (E1E7I) XP® Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Myeloperoxidase (E1E7I) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Myeloperoxidase (E1E7I) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded HL-60 (left) and HeLa (right) cell pellets using Myeloperoxidase (E1E7I) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HL-60 cells (left, positive) or HeLa cells (right, negative) using Myeloperoxidase (E1E7I) XP® Rabbit mAb #14569 (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
To Purchase # 88757
Cat. # Size Qty. Price
88757SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 60, 80-90
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #14569. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #14569.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Myeloperoxidase (E1E7I) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total myeloperoxidase protein. This antibody recognizes the full-length and heavy chain subunits of human myeloperoxidase protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro734 of human myeloperoxidase protein.

Background

Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells (5). MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections (6). Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions (1).
  1. Klebanoff, S.J. (2005) J Leukoc Biol 77, 598-625.
  2. Chang, K.S. et al. (1986) Blood 68, 1411-4.
  3. Yamada, M. et al. (1987) Arch Biochem Biophys 255, 147-55.
  4. Morishita, K. et al. (1987) J Biol Chem 262, 3844-51.
  5. Weil, S.C. et al. (1987) Proc Natl Acad Sci USA 84, 2057-61.
  6. Aratani, Y. et al. (2002) J Infect Dis 185, 1833-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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