Cat. # | Size | Qty. | Price |
---|---|---|---|
64038S | 100 µl |
|
REACTIVITY | H R |
SENSITIVITY | Endogenous |
MW (kDa) | 140-210 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1932 of human MYH7 protein.
Myosin heavy chain 7 (MYH7), also known as β-myosin heavy chain, is a type I slow-twitch fiber expressed in muscle fibers as well as heart ventricles and is essential for muscle contraction (1). MYH7 is composed of a globular head domain at the N-terminus, a hinge region, and an α-helical coil-coil rod domain (2). MYH7 forms a hexameric complex with two MYH7, two myosin light chain 2 (MYL2), and two myosin light chain 3 (MYL3) (3). The myosin-binding sites on actin filaments become exposed by the binding of Ca+, which leads to actin-myosin junction formation and the ability to glide along the actin base. The complexes become detached in the presence of ATP, and the next cycle of contraction is initiated when ATPase activity of the head domain hydrolyzes ATP (4). Mutations in the MYH7 gene can lead to hypertrophic cardiomyopathy (HCM), dilated CM (DCM), and left ventricle non-compaction (LVNC) (5).
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