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Myosin II Isoform Antibody Sampler Kit #5144

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    Product Information

    Product Description

    The Myosin II Isoform Antibody Sampler Kit is an economical way to examine the total protein levels of myosin II isoforms a, b, and c. The kit includes enough primary and secondary antibodies to perform four Western blot experiments.

    Background

    Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).

    Alternate Names

    cellular myosin heavy chain; cellular myosin heavy chain type B; myh10; myh14; myh9; myosin heavy chain 14; myosin heavy chain 9; NMHC; NMMHCA; NMMHCB; NMMHCC; nonmuscle myosin type A; nonmuscle myosin type B

    For Research Use Only. Not for Use in Diagnostic Procedures.
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