Render Target: STATIC
Render Timestamp: 2024-10-14T11:00:18.088Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:59:25.894
Product last modified at: 2024-09-30T08:02:02.966Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NAT10 (E5U9W) Rabbit mAb #30413

Filter:
  • WB

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 120
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NAT10 (E5U9W) Rabbit mAb recognizes endogenous levels of total NAT10 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu228 of human NAT10 protein.

    Background

    N-acetyltransferase 10 (NAT10), also known as human N-acetyltransferase-like protein (hALP), is an acetyltransferase that increases telomerase activity through the activation of the TERT promoter (1). NAT10 localizes to the midbody in late phase mitosis to help achieve cytokinesis (2). It has been found that DNA damage can actually increase NAT10-dependent acetylation of alpha-tubulin, resulting in increased stability of the protein (2,3). This increased stability of microtubules has been linked to the sequestration of the nuclear import factor transportin-1 in the premature aging disease, progeria (4,5). NAT10 can also function as an acetyltransferase for both rRNA and mRNA (6-8). The acetylation of the 18 S rRNA helps in rRNA processing and ribosome biogenesis, and this process can be disrupted by opposing activity of the deacetylase SirT1 in response to stress (6,9). mRNA acetylation by NAT10 plays a role in translation efficiency and mRNA stability (7,8). NAT10 is overexpressed in many different cancer types, making it a good potential therapeutic target (10-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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