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NCBP1/CBP80 Antibody #9983

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Inquiry Info. # 9983

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 80
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NCBP1/CBP80 Antibody recognizes endogenous levels of total NCBP1/CBP80 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val452 of human NCBP1/CBP80 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Nuclear cap-binding protein subunit 1 (NCBP1), also known as cap-binding protein 80 (CBP80), plays a role in nuclear pre-mRNA splicing (1,2). It has also been shown to function in the nonsense-mediated decay (NMD) of mRNAs where translation is prematurely terminated (3). NCBP1/CBP80 increases the efficiency of NMD by promoting the interaction of two active NMD components Upf1 and Upf2 (4).

    Alternate Names

    80 kDa nuclear cap-binding protein; CBP80; MGC2087; NCBP; NCBP 80 kDa subunit; NCBP1; nuclear cap binding protein subunit 1; nuclear cap binding protein subunit 1, 80kDa; Nuclear cap-binding protein subunit 1; Sto1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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