Neurofascin 155 (D7B6O) Rabbit mAb (BSA and Azide Free) #48813
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140-155 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #15035
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Alternative splicing produces several neurofascin isoforms that differ in temporal and spatial expression. Neurofascin 186 is expressed in axons where it is concentrated at the nodes. Research studies indicate that neurofascin 186 is responsible for nodal assembly and clustering of sodium channels (9). Neurofascin 155 is expressed in glial cells and is localized to myelin paranodes. Interactions between neurofascin 155 and the contactin-associated protein (Caspr) tether the myelin sheath to the axon (10). N-linked glycosylation results in two forms of neurofascin 155 (high and low) that are differentially expressed during development (11).
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- Salzer, J.L. (1997) Neuron 18, 843-6.
- Waxman, S.G. et al. (1989) Proc Natl Acad Sci U S A 86, 1406-10.
- Ritchie, J.M. (1992) Trends Neurosci 15, 345-51.
- Berghs, S. et al. (2000) J Cell Biol 151, 985-1002.
- Zhou, D. et al. (1998) J Cell Biol 143, 1295-304.
- Davis, J.Q. et al. (1996) J Cell Biol 135, 1355-67.
- Ratcliffe, C.F. et al. (2001) J Cell Biol 154, 427-34.
- Thaxton, C. et al. (2011) Neuron 69, 244-57.
- Charles, P. et al. (2002) Curr Biol 12, 217-20.
- Pomicter, A.D. et al. (2010) Brain 133, 389-405.
Limited Uses
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