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NFI-A (E6Z1R) Rabbit Monoclonal Antibody #69375

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  • WB
  • IF
  • ChIP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NFI-A (E6Z1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total NFI-A protein. Based on sequence homology, this antibody is not predicted to cross-react with other NFI family members.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro430 of human NFI-A protein.

    Background

    NFI-A belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). NFI-A activates cell-type-specific enhancers and facilitates the binding of PPARγ, the master transcriptional regulator of adipogenesis, to control the brown fat gene program (4). Additionally, NFI-A represses the expression of Myod1, the master transcriptional regulator of myogenesis, thus actively suppressing the myogenic gene program (5).

    Alternate Names

    BRMUTD; CCAAT-box-binding transcription factor; CTF; DKFZp434L0422; DKFZp686J23256; FLJ39164; KIAA1439; NF-I/A; NF1-A; NFI-A; NFI-L; NFIA; Nuclear factor 1 A-type; Nuclear factor 1/A; nuclear factor I A; Nuclear factor I/A; TGGCA-binding protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
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