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Render Timestamp: 2024-10-11T10:00:18.066Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:58:59.386
Product last modified at: 2024-09-30T08:01:19.577Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. NFI-B (E5L7U) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NFI-B (E5L7U) Rabbit mAb #24903

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 50-75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NFI-B (E5L7U) Rabbit mAb recognizes endogenous levels of total NFI-B protein. Based on antigen sequence, the antibody is predicted to detect isoform 1 and isoforms 4-6 of NFI-B. Based on sequence homology, this antibody is not predicted to cross-react with other NFI family members. Species cross-reactivity for IF-IC is human only.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro404 of human NFI-B protein.

    Background

    NFI-B belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). Research studies have demonstrated that NFI-B plays a crucial role in different aspects of tumor development, acting as an oncogene or tumor suppressor in different types of tumors and participating in tumor-associated fusion gene formation (4-7). Moreover, NFI-B has been shown to promote epithelial-mesenchymal transition and metastatic ability of cancer cells through a number of mechanisms, including upregulating SNAI1 expression in colorectal cancer (8), stabilizing chromatin accessibility in small cell lung cancer (9), and modulating the ZEB1 axis in melanoma (10).

    Database Links

    UniProt ID: O00712


    Entrez-Gene Id: 4781


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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