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  3. NINJ1 (F2Y6T) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NINJ1 (F2Y6T) Rabbit Monoclonal Antibody #49761

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 18
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NINJ1 (F2Y6T) Rabbit Monoclonal Antibody recognizes endogenous levels of total NINJ1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length human NINJ1 protein. This antibody recognizes residues surrounding Ala42 of human NINJ1 protein.

    Background

    Ninjurin 1 (NINJ1) is a transmembrane protein initially identified for its involvement in nerve regeneration and cell adhesion (1). Research studies have also highlighted its primary function as a mediator of plasma membrane rupture (PMR) during various programmed cell death pathways, including pyroptosis, necroptosis, apoptosis, and ferroptosis, or in response to osmotic stress and mechanical tension (2). In response to death stimuli, NINJ1 oligomerizes and forms ring-like or filamentous structures on the plasma membrane, which lead to irreversible membrane damage, cell lysis, the release of damage-associated molecular patterns (DAMPs), and an inflammatory response in the extracellular environment (3-6). NINJ1 is also involved in leukocyte migration through facilitating cell-to-cell interactions between immune and endothelial cells, promoting the transendothelial migration of macrophages, and regulating Toll-like receptor 4 (TLR4) signaling (7,8). NINJ1-mediated transmigration of inflammatory cells across the blood-brain barrier contributes to neuroinflammation in experimental autoimmune encephalomyelitis (9), and NINJ1-mediated PMR of pyroptotic endothelial cells exacerbates blood-brain barrier destruction in traumatic brain injury (10). The role of NINJ1 in cancer biology is multifaceted and appears to be context-dependent, with evidence supporting both tumor-promoting (11-13) and tumor-suppressing functions (14-17).
    1. Araki, T. and Milbrandt, J. (1996) Neuron 17, 353-61.
    2. Zhu, L. and Xu, Y. (2025) Front Immunol 16, 1519519.
    3. Kayagaki, N. et al. (2021) Nature 591, 131-136.
    4. Degen, M. et al. (2023) Nature 618, 1065-1071.
    5. Ramos, S. et al. (2024) EMBO J 43, 1164-1186.
    6. David, L. et al. (2024) Cell 187, 2224-2235.e16.
    7. Jennewein, C. et al. (2015) Am J Respir Cell Mol Biol 53, 656-63.
    8. Wu, Z. et al. (2024) Adv Sci (Weinh) 11, e2306237.
    9. Ifergan, I. et al. (2011) Ann Neurol 70, 751-63.
    10. Zheng, X.B. et al. (2025) Cell Death Discov 11, 69.
    11. Park, J. et al. (2017) Anticancer Res 37, 1687-1696.
    12. Hyun, S.Y. et al. (2022) J Exp Clin Cancer Res 41, 133.
    13. Song, C.H. et al. (2025) Int J Cancer 156, 826-839.
    14. Toyama, T. et al. (2004) J Hepatol 41, 637-43.
    15. Jang, Y.S. et al. (2016) Int J Cancer 139, 383-95.
    16. Woo, J.K. et al. (2016) Oncotarget 7, 29592-604.
    17. Chen, S.Y. et al. (2024) Cell Death Dis 15, 755.

    Alternate Names

    Nerve injury-induced protein 1; nerve injury-induced protein-1; NIN1; NINJ1; NINJURIN; ninjurin 1; Ninjurin-1; OTTHUMP00000021670

    Database Links

    UniProt ID: Q92982


    Entrez-Gene Id: 4814


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