Render Target: STATIC
Render Timestamp: 2024-11-06T10:31:54.463Z
Commit: 642d75590e907c0f7dfc7c6e3b846bcc0b02197c
XML generation date: 2024-09-30 01:53:43.462
Product last modified at: 2024-10-16T14:15:09.451Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NIPSNAP1 (D1Y6S) Rabbit mAb #13226

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 29
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NIPSNAP1 (D1Y6S) Rabbit mAb recognizes endogenous levels of total NIPSNAP1 protein. This antibody does not cross-react with NIPSNAP2 (GBAS) protein. This antibody recognizes the mature 29 kDa form of NIPSNAP1 as described in Okuda-Ashitaka, E. et al. (2012).

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg211 of human NIPSNAP1 protein.

    Background

    4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) is a member of a highly conserved family of proteins whose functions include the regulation of channel activity, mitochondrial function and cognitive function.

    Interaction of NIPSNAP1 with the putative oncogene Ca2+-selective transient receptor potential vanilloid channel 6 (TRPV6) inhibits channel function at the cell membrane (1,2). In prostate cancer cells, alterations in chromatin structure that result in corresponding NIPSNAP1 gene inactivation have been implicated in the malignant phenotype (3).

    In mouse brain, NIPSNAP has been shown to interact with mitochondrial amyloid precursor protein (APP), which may facilitate the effect of APP on mitochondrial function. (4). NIPSNAP1 expression is also altered in the brains of phenylketonuria (PKU) mice, implying a role for NIPSNAP1 in PKU-related cognitive impairment (5). NIPSNAP1 has also been implicated in pain transmission through its interaction with the neuropeptide nocistatin (NST) in mouse spinal cord (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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