Render Target: STATIC
Render Timestamp: 2024-12-11T12:22:07.374Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-11-20 16:46:06.181
Product last modified at: 2024-11-21T08:01:03.202Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Non-phospho-Src (Tyr416) (7G9) Mouse mAb #2102

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Mouse IgG2b
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Non-phospho-Src (Tyr416) (7G9) Mouse mAb detects endogenous levels of Src only when dephosphorylated at tyrosine 416. The antibody cross-reacts with other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites.

    Species Reactivity:

    Human, Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Chicken, Xenopus

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr419 of human Src.

    Background

    The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
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