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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Notch1 (D1E11) XP® Rabbit mAb 3608 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation
H M R 120, 300 Rabbit IgG
Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb 4147 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R 110 Rabbit IgG
RBPSUH (D10A4) XP® Rabbit mAb 5313 20 µl
Western Blotting Immunohistochemistry Chromatin Immunoprecipitation
H M R Mk 61 Rabbit IgG
MAML1 (D3K7B) Rabbit mAb 12166 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R 130 Rabbit IgG
c-Myc (D3N8F) Rabbit mAb 13987 20 µl
Western Blotting Immunofluorescence Chromatin Immunoprecipitation
H M R Mk 57-65 Rabbit IgG
p21 Waf1/Cip1 (12D1) Rabbit mAb 2947 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H Mk 21 Rabbit IgG
HES1 (D6P2U) Rabbit mAb 11988 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M R Mk 30 Rabbit IgG
Cyclin D3 (DCS22) Mouse mAb 2936 20 µl
Western Blotting Immunohistochemistry
H M R 31 Mouse IgG1
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
Horse 

Product Description

The Notch Activated Targets Antibody Sampler Kit provides an economical means of detecting target proteins of activated Notch. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.


Specificity / Sensitivity

Notch1 (D1E11) XP® Rabbit mAb detects intracellular epitopes between 2400 and 2500 amino acids of human Notch1. It recognizes both the full-length (~300 kDa) and the NTM region (~120 kDa). The antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases. Cleaved Notch1 (V1744) (D3B8) Rabbit mAb detects endogenous levels of the Notch1 intracellular domain (NICD) only when released by cleavage between Gly1753 and Val1754 (equivalent to Gly1743/Val1744 of murine notch1). The antibody does not recognize full-length Notch1 or Notch1 cleaved at other positions. The size of the NICD varies among cell lines due to mutations in the Notch1 C-terminus. RBPSUH (D10A4) XP® Rabbit mAb recognizes endogenous levels of total RBPSUH protein. MAML1 (D3K7B) Rabbit mAb recognizes endogenous levels of total MAML1 protein. This antibody does not detect MAML2 or MAML3. c-Myc (D3N8F) Rabbit mAb recognizes endogenous levels of total c-Myc protein. p21 Waf1/Cip1 (12D1) Rabbit mAb detects endogenous levels of total p21 protein. The antibody does not cross-react with other CDK inhibitors. HES1 (D6P2U) Rabbit mAb recognizes endogenous levels of total HES1 protein. Cyclin D3 (DCS22) Mouse mAb detects endogenous levels of total cyclin D3 protein. The antibody does not cross-react with cyclin D1 or cyclin D2. 


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2438 of human Notch1, the Val1754 cleavage site in human Notch1 (equivalent to Val1744 in mouse Notch1), residues surrounding Gln110 of human RBPSUH protein, residues surrounding Asp269 of human MAML1 protein, a central region within human c-Myc protein, residues near the carboxy-terminus of human p21,  recombinant protein specific to human HES1 protein, residues 241-260 of  recombinant human cyclin D3. Antibodies are purified by protein A and peptide affinity chromatography.

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4).  RBPSUH (Recombining Binding Protein, SUppressor of Hairless), is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. Binding of Notch with RBPSUH activates a transcription activation complex that includes Mastermind-like (MAML) proteins, leading to transcriptional activation of Notch target genes (5-7). The NICD binds and activates c-Myc which functions as a transcriptional regulator with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (8).  The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. The NICD-RBPSUH complex binds and activates p21 for transcription (15).  HES1 (Hairy and Enhancer of Split 1) is one of seven members of the HES family of basic helix-loop-helix (bHLH) transcription factors that is particularly well known as a repressive mediator of the canonical Notch signaling pathway (10). HES1 plays a key role in mediating Notch-dependent T cell lineage commitment (11), and has been reported to be an essential mediator of Notch-induced T cell acute lymphoblastic leukemia (T-ALL) (11,12). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (13).  Transcription of cyclin D is in part regulated by the NICD binding to the promoter region of cyclin D (14).


1.  Li, X. and von Boehmer, H. (2011) ISRN Hematol 2011, 921706.

2.  Niimi, H. et al. (2007) J Cell Biol 176, 695-707.

3.  Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.

4.  Wu, L. et al. (2002) Mol Cell Biol 22, 7688-700.

5.  Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.

6.  Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.

7.  Lin, S.E. et al. (2002) J Biol Chem 277, 50612-20.

8.  Schroeter, E.H. et al. (1998) Nature 393, 382-6.

9.  Kitagawa, M. et al. (2001) Mol Cell Biol 21, 4337-46.

10.  Flores-Rozas, H. et al. (1994) Proc Natl Acad Sci U S A 91, 8655-9.

11.  Kobayashi, T. and Kageyama, R. (2010) Genes Cells 15, 689-98.

12.  Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.

13.  Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.

14.  Wendorff, A.A. et al. (2010) Immunity 33, 671-84.

15.  Espinosa, L. et al. (2010) Cancer Cell 18, 268-81.


Entrez-Gene Id 896 , 3280 , 9794 , 4609 , 4851 , 1026 , 3516
Swiss-Prot Acc. P30281 , Q14469 , Q92585 , P01106 , P46531 , P38936 , Q06330


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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Notch Activated Targets Antibody Sampler Kit