Notch1 (5B5) Rat mAb #3447
- WB
- IP
Supporting Data
| REACTIVITY | H M R B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120, 300 |
| Source/Isotype | Rat IgG2b |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Notch1 (5B5) Rat mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) protein and the transmembrane/intracellular region NTM (~120 kDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). The antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases.
Species Reactivity:
Human, Mouse, Rat, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of Notch1.
Background
Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4).
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