NRK1 (F4F8L) Rabbit mAb #29786

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	23
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NRK1 (F4F8L) Rabbit mAb recognizes endogenous levels of total NRK1 protein. This antibody does not cross-react with NRK2 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp48 of human NRK1 protein.

Background

Nicotinamide riboside (NR) is converted to NAD precursor nicotinamide mononucleotide (NMN) by nicotinamide riboside kinase 1 and 2 (NRK1 and NRK2), a function shared by yeast Nrk1 (1). Cytosolic NRK1, encoded by the NMRK1 gene, is expressed in most tissues and enriched in liver and kidney, while NRK2 expression is limited to muscle (2). Primary hepatocytes from NRK1 mice were used to demonstrate that NRK1 is essential for utilization of exogenous NR or NMN to produce NAD⁺ (2). Tissue-specific expression of NAD synthesis enzymes can drive cancer cell survival and treatment strategies. Cancer cells can arise in tissues with variable expression of rate-limiting enzymes for NAD synthesis, including nicotinate phosphoribosyltransferase (NAPRT) or nicotinamide phosphoribosyltransferase (NAMPT) (3). In instances where this expression is low, NRK1-driven NAD synthesis is key for cancer cell survival and may bypass inhibitors of the other NAD⁺ pathways (3).

Database Links

UniProt ID: Q9NWW6

Entrez-Gene Id: 54981

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For Research Use Only. Not For Use In Diagnostic Procedures.

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