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87661
NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free) #87661

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  1. WB
  2. IHC
Western Blotting Image 1: NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from HT-29 and Huh7 cells, and rat eye using NT5E/CD73 (D7F9A) Rabbit mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 2: NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with cobalt chloride (CoCl2, 0.1 mM, 16 hr; +), using NT5E/CD73 (D7F9A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NT5E/CD73 (D7F9A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse liver using NT5E/CD73 (D7F9A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded rat kidney using NT5E/CD73 (D7F9A) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 87661
Cat. # Size Qty. Price
87661SF
100 µg $ 746

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 70
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #13160. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #13160.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

NT5E/CD73 (D7F9A) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total NT5E/CD73 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein.

Background

Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).
  1. Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.
  2. Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.
  3. Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.
  4. Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.
  5. Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.
  6. Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.
  7. Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.
  8. Zylka, M.J. (2011) Trends Mol Med 17, 188-96.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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