NYAP1 (E6D4S) Rabbit mAb #35227

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	105
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NYAP1 (E6D4S) Rabbit mAb recognizes endogenous levels of total NYAP1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser369 of human NYAP1 protein.

Background

Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adaptor 1 (NYAP1) is a member of the Nyap family of phosphoproteins, including NYAP1, NYAP2, and Myosin16/NYAP3. Upon stimulation by contactin5, NYAPs are phosphorylated by Fyn. Once phosphorylated, NYAP1, as well as other members of this family, interacts with PI3K p85 and active PI3K, Akt, and Rac1. The interaction of NYAP1 with WAVE complex mediates remodeling of the actin cytoskeleton after activation by PI3K-produced PIP3 and Rac1 (1). The phosphorylation of NYAP1 directly contributes to normal neuronal migration and morphogenesis of neurons, acting downstream of Fyn, which is a non-receptor protein-tyrosine kinase that is a member of the Src kinase family (2). The overexpression of NYAP1 promotes neurite branching and elongation at early neuronal migration compared to the NYAP1-KO model. The lack of NYAP1 induces thinner leading neurites affecting the multibipolar morphology of neurons (2).

Database Links

UniProt ID: Q6ZVC0

Entrez-Gene Id: 222950

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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