Render Target: STATIC
Render Timestamp: 2024-10-11T10:04:31.691Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-25 16:36:59.478
Product last modified at: 2024-09-28T08:00:11.297Z
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. OCA-T2 (F5A7Z) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

OCA-T2 (F5A7Z) Rabbit mAb #48122

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    OCA-T2 (F5A7Z) Rabbit mAb recognizes endogenous levels of total OCA-T2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human OCA-T2 protein.

    Background

    OCA-T2 (COLCA2, POU2AF3) and its paralog OCA-T1 (C11orf53) were first identified by single-cell RNA sequencing (scRNAseq) to be co-expressed with the transcription factor POU2F3, in specialized epithelial cells called tuft cells (1). Tuft cells exist as solitary chemosensory cells within mucosal epithelium and serve to coordinate interactions between immune and neuronal cells (2). Systematic gene knockdown experiments demonstrated chromatin-level interactions between OCA-T1, OCA-T2, and POU2F3 that appeared essential for tuft cell lineage determination (1). Notably, the oncogenic transformation of tuft cells has been identified as a defining feature of a distinct small cell lung cancer (SCLC) subtype known as SCLC-P, defined by their dependence on POU2F3 expression (3). In vitro studies showed that targeted knockdown of OCA-T1 and OCA-T2 suppressed growth of cultured SCLC-P cell lines, supporting a role for OCA-T1 and OCA-T2 in SCLC (4). Subsequently, it has been observed that gene fusions between EWSR1 or FUS and OCA-T2 are defining features of recently discovered aggressive sarcoma subtypes (5-7).

    Database Links

    UniProt ID: A8K830


    Entrez-Gene Id: 120376


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.