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Olfm4 (D6Y5A) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Olfm4 (D6Y5A) XP® Rabbit mAb (BSA and Azide Free) #25426

Citations (0)
  1. WB
  2. IHC
  3. IF
Western blot analysis of extracts from mouse small intestine (positive), mouse spleen (positive), and mouse colon (negative) using Olfm4 (D6Y5A) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded wild-type (left) and Olfm4 knockout (right) mouse spleen using Olfm4 (D6Y5A) XP® Rabbit mAb. (Tissue courtesy of Dr. Wenli Lui and Dr. Griffin P. Rodgers, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded normal mouse small intestine (left) and colon (right) using Olfm4 (D6Y5A) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded APC (Min/+) mouse intestinal adenoma using Olfm4 (D6Y5A) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mouse small intestine at low (left) and high magnification (right) using Olfm4 (D6Y5A) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the cells were then labeled using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #36110 (red) and CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate) #81143 (cyan pseudocolor). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
To Purchase # 25426
Cat. # Size Qty. Price
100 µg

Supporting Data

MW (kDa) 85-90, 70
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&T-CUT&Tag
  • DB-Dot Blot
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #39141. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.


Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #39141


Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Olfm4 (D6Y5A) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total mouse Olfm4 protein.

Species Reactivity:


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala85 of mouse Olfm4 protein.


Olfactomedin-4 (OLFM4, hGC-1) is a member of the Olfactomedin family, a small group of extracellular proteins defined by the presence of a conserved "Olfactomedin domain" that is thought to facilitate protein-protein interactions (1). OLFM4 is a secreted glycoprotein, which forms disulfide bond-mediated oligomers, and is thought to mediate cell adhesion through its interactions with extracellular matrix proteins such as lectins (2). Human OLFM4 was first cloned from myeloid cells (3) and is expressed in a distinct subset of neutrophils, though the functional significance of this differential expression pattern remains unclear (4). Among normal tissues, the expression of OLFM4 protein is most abundant in intestinal crypts (5), where it has garnered attention as a possible marker of intestinal stem cells (6). Notably, OLFM4 expression is markedly increased in several tumor types, including colorectal, gastric, pancreas, lung, and breast (reviewed in [1]). Furthermore, research studies show that the expression levels of OLFM4 vary in relation to the severity and/or differentiation status of multiple tumor types (1, 6-8), leading to the suggestion that OLFM4 may have utility as a prognostic marker in some cancer patients (9).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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