Olfm4 (D6Y5A) XP® Rabbit mAb (BSA and Azide Free) #25426
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 85-90, 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Olfm4 (D6Y5A) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total mouse Olfm4 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala85 of mouse Olfm4 protein.
Background
Olfactomedin-4 (OLFM4, hGC-1) is a member of the Olfactomedin family, a small group of extracellular proteins defined by the presence of a conserved "Olfactomedin domain" that is thought to facilitate protein-protein interactions (1). OLFM4 is a secreted glycoprotein, which forms disulfide bond-mediated oligomers, and is thought to mediate cell adhesion through its interactions with extracellular matrix proteins such as lectins (2). Human OLFM4 was first cloned from myeloid cells (3) and is expressed in a distinct subset of neutrophils, though the functional significance of this differential expression pattern remains unclear (4). Among normal tissues, the expression of OLFM4 protein is most abundant in intestinal crypts (5), where it has garnered attention as a possible marker of intestinal stem cells (6). Notably, OLFM4 expression is markedly increased in several tumor types, including colorectal, gastric, pancreas, lung, and breast (reviewed in [1]). Furthermore, research studies show that the expression levels of OLFM4 vary in relation to the severity and/or differentiation status of multiple tumor types (1, 6-8), leading to the suggestion that OLFM4 may have utility as a prognostic marker in some cancer patients (9).
- Yu, L. et al. (2011) Neoplasma 58, 9-13.
- Liu, W. et al. (2006) Exp Cell Res 312, 1785-97.
- Zhang, J. et al. (2002) Gene 283, 83-93.
- Welin, A. et al. (2013) PLoS One 8, e69575.
- van der Flier, L.G. et al. (2009) Gastroenterology 137, 15-7.
- Liu, W. et al. (2007) Histopathology 51, 157-65.
- Liu, W. et al. (2008) Clin Cancer Res 14, 1041-9.
- Besson, D. et al. (2011) Mol Cell Proteomics 10, M111.009712.
- Seko, N. et al. (2010) Exp Ther Med 1, 73-8.
Limited Uses
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